The distribution of Mas-related G protein-coupled receptor A in cerebrospinal fluid-contacting nucleus of normal rats and its up-regulation in neuropathic pain / 生理学报

This study was aimed to observe the distribution of Mas-related G protein-coupled receptor A (MrgA) in cerebrospinal fluid (CSF)-contacting nucleus of normal rats and its expression in neuropathic pain, and to provide morphological evidence for CSF-contacting nucleus to participate in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of the sciatic nerve was made in Sprague-Dawley rats. The thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. The expressions of MrgA in the CSF-contacting nucleus were examined by double labeling with immunofluorescent staining. The results showed that on the 5th, 7th, 10th and 14th days, the values of MWT and TWL in CCI group were all lower than those in sham group (P < 0.05). MrgA was found to be distributed in CSF-contacting nucleus of normal rats; and the expression was markedly up-regulated in rats at the peak of neuropathic pain. Our data suggest that CSF-contacting nucleus may participate in neuropathic pain through the MrgA-mediated signaling pathway.

Effect of psoralen on rat osteoblasts injuries induced by TCP wear particles in vitro and its mechanism / 中国应用生理学杂志

To investigate the effect and mechanism of psoralen on calvarial osteoblasts injuries caused by tricalcium phosphate (TCP) wear particles in vitro. Primary osteoblasts were obtained from the calvaria of neonatal SD rat by the series of digestion and were identified with ALP staining. Calvarial osteoblasts were treated with TCP wear particles for 48 h to establish the in vitro model of osteoblasts injuries. The rat osteoblasts were randomly divided into control group, TCP wear particles (0.1 mg/ml) group, psoralen treated (at the concentrations of 10, 10, 10 mol/L) groups. WST assay and the flow cytometry were used to detect the cell viability of osteoblasts and apoptosis, respectively. Chemical colorimetry was performed to examine ALP activity of osteobalsts. When the osteoblasts were treated for 14 day, mineral nodules formation was observed with alizarin red S staining. Western blot was applied to examine protein expressions of glucose regulated protein78/94(GRP78/94), inositol dependent enzyme 1 alpha (IREα), spliced X-box binding protein 1 (XBP1s) and phosphorylated c-Jun N-terminal kinase (p-JNK) in calvarial osteoblasts. Compared with control group, the cell viability of osteoblasts, ALP activity and mineral nodules formation in TCP group were decreased significantly (P＜0.05), while the percentage of apoptosis and protein expressions of GRP78/94, IRE1α, XBP1 and p-JNK were obviously increased in calvarial osteoblasts (P＜0.05). Compared with TCP group, the injuries of calvarial osteoblasts and cell apoptosis in psoralen treated groups were obviously decreased (P＜0.05), and the expression levels of GRP78/94, IRE1α, XBP1 and p-JNK were down-regulated remarkably (P＜0.05). Psoralen prevents osteoblasts injuries caused by TCP wear particles through IRE1α-XBP1s-JNK signaling pathway activation.

Epidemic situation of malaria in Dalian City from 2005 to 2016 / 中国血吸虫病防治杂志

Objective To describe and analyze the epidemiological characteristics of malaria in Dalian City, so as to provide the evidence for adjusting and formulating malaria control strategy and measures, being in accordance with the practical situation in Dalian City. Methods The data of malaria cases in Dalian City from 2005 to 2016 were collected from China Diseases Surveillance Information Reporting Management System (DSIRMS), to describe and analyze the epidemiologic characteristics of malaria in Dalian City. Results A total of 143 malaria cases were reported in Dalian from 2005 to 2016, including 115 laboratory diagnosed cases, 27 clinically diagnosed cases, and 1 suspected diagnosed case. Among the 115 laboratory diagnosed patients, 65 patients were infected with Plasmodium falciparum, 19 were infected with P. vivax, and 15 had mixed infection, 4 were infected with P. ovale, 3 were infected with P. malariae, and 9 were unclassified. All the cases were imported, 127 were imported from Africa, accounting for 88.81%, mainly were imported from Equatorial Guinea, Liberia, Ghana and Nigeria. Most of the cases were imported from May to October, accounting for 58.74% (84/143), and the imported cases emerged a small peak in August. The ratio of males to females was 10∶1, and the median age was 42 years. Among the total cases, fishermen, migrant workers, and work-man accounted for 55.24% (79/143). Seventy-five patients lived in rural districts, and 68 patients lived in urban districts. The median interval from backing to China to disease onset was 8 days. The proportion of disease onset within one month after backing to China was 76.27% (90/118). The median interval from disease onset to diagnosis was 4 days, and the longest interval was 71 days. Totally 141 patients were cured and 2 died. Conclusion In order to avoid malaria death, we should strengthen the malaria prevention and control, especially in rural districts, enhance the training for professionals in the county health facilities, and the surveillance and health education for those who are returned from malaria-endemic areas.

Epidemic situation of malaria in Dalian City from 2005 to 2016 / 中国血吸虫病防治杂志

Objective To describe and analyze the epidemiological characteristics of malaria in Dalian City, so as to provide the evidence for adjusting and formulating malaria control strategy and measures, being in accordance with the practical situation in Dalian City. Methods The data of malaria cases in Dalian City from 2005 to 2016 were collected from China Diseases Surveillance Information Reporting Management System (DSIRMS), to describe and analyze the epidemiologic characteristics of malaria in Dalian City. Results A total of 143 malaria cases were reported in Dalian from 2005 to 2016, including 115 laboratory diagnosed cases, 27 clinically diagnosed cases, and 1 suspected diagnosed case. Among the 115 laboratory diagnosed patients, 65 patients were infected with Plasmodium falciparum, 19 were infected with P. vivax, and 15 had mixed infection, 4 were infected with P. ovale, 3 were infected with P. malariae, and 9 were unclassified. All the cases were imported, 127 were imported from Africa, accounting for 88.81%, mainly were imported from Equatorial Guinea, Liberia, Ghana and Nigeria. Most of the cases were imported from May to October, accounting for 58.74% (84/143), and the imported cases emerged a small peak in August. The ratio of males to females was 10∶1, and the median age was 42 years. Among the total cases, fishermen, migrant workers, and work-man accounted for 55.24% (79/143). Seventy-five patients lived in rural districts, and 68 patients lived in urban districts. The median interval from backing to China to disease onset was 8 days. The proportion of disease onset within one month after backing to China was 76.27% (90/118). The median interval from disease onset to diagnosis was 4 days, and the longest interval was 71 days. Totally 141 patients were cured and 2 died. Conclusion In order to avoid malaria death, we should strengthen the malaria prevention and control, especially in rural districts, enhance the training for professionals in the county health facilities, and the surveillance and health education for those who are returned from malaria-endemic areas.

Analysis of Pelvic Fracture Healing Status in 198 Cases / 法医学杂志

Objective To analyze the relationship between the number of pelvic ring fractures, the location of fracture and Tile type of pelvic fracture and pelvic fracture healing status. To discuss how to understand the pelvic malunion, severe pelvic malunion and bony pelvis deformity （destruction） in Classification of the Impairment Related to Injury. Methods One hundred and ninety-eight cases of evaluation of body impairment class of pelvic fracture caused by traffic accident accepted by Zhongcheng Institute of Forensic Science in 2016 were collected. The cases were statistically analyzed in terms of the distribution of the number of pelvic ring fracture, the location of pelvic ring fracture and Tile type of pelvic fracture in various healing statuses. Results There were 16 cases without pelvic ring fracture, 18 cases of single pelvic ring fracture, 91 cases of 2 pelvic ring fractures, 73 cases of 3 or more pelvic ring fractures, 136 cases of anterior or posterior pelvic ring fractures, 46 cases of simultaneous anterior and posterior ring fractures. There were 34 cases of Tile A type, 130 cases of Tile B type and 24 cases of Tile C type. Statistical analysis results showed that, the differences in distribution of the number of pelvic ring fracture, the location of pelvic ring fracture and Tile type of pelvic ring fracture in various healing statuses had statistical significance （P<0.05）. Conclusion In determination of the healing status of pelvic fracture, preliminary judgment can be made by using the number of pelvic ring fracture, status of pelvic ring fracture and Tile type of pelvic fracture. Caution is required when dealing with single pelvic fractures （including dislocations）, and determining the destruction of bony pelvis.

Verification of skin paste electrodes used in wireless polysomnography / 北京大学学报(医学版)

OBJECTIVE@#To explore an electrode suitable for wireless portable sleep monitoring equipment and analyze the result of the signals of electrooculogram (EOG) and electroencephalography (EEG) collected by this kind of flexible electrodes.@*METHODS@#The flexible electrodes were prepared by microelectromechanical systems (MEMS) technology. This kind of electrodes consisted parylene, chromium, and gold. Parylene, the flexible substrate of this kind of flexible electrodes, was of biocompatibility. Between parylene and gold there was an adhesion layer of chromium, which connected parylene and gold tightly. Then the flexible electrodes were stuck to medical adhesive tape. The electrodes were designed and made into a grid to make sure that the medical adhesive tape could tape on the skin tightly, so that the contact impedance between the electrodes and the skin would be reduced. Then the alternating current impedance of the electrode were tested by the CHI660E electrochemical workstation after the electrode was achieved. To make sure that this kind of electrodes could be used in EOG monitoring, the electrodes were connected to a wireless signal acquisition suite containing special biological signal acquisition and digital processing chip to gather different sites around the eyes and the electrical signals of different directions of the eye movements, then analyzed the signal-to-noise ratio of the EOG. At the end, the Philips A6 polysomnography was used to compare the noise amplitude of the EEG signals collected by the flexible electrode and the gold cup electrode.@*RESULTS@#The electrodes stuck to the skin tightly, and these electrodes could collect signals that we wanted while the experiment was performed. The alternating current impedance of the flexible electrode was between 4 kΩ and 13 kΩ while with the frequency of alternating current under 100 Hz, most EEG signal frequencies were at this range. The EOG signals collected by the flexible electrodes were in line with the clinical requirements. The noise amplitude of EEG signals collected by the flexible electrodes was lower than that of the electrical signals collected by the gold cup electrodes.@*CONCLUSION@#The flexible electrode could be taken into consideration as an alternative electrode for monitoring EOG and EEG signals, and the wireless portable sleep monitoring devices are to be further developed in the future.

Study on photosynthesis and physiological characteristics of Acanthopanax giraldii on different light intensity / 中国中药杂志

This article is aimed to study the response mechanism of Acanthopanax giraldii on different shading intensity to guide its artificial cultivation. The cultivated A. giraldii in Maoxian was used as the research object, set up different shading treatment groups, analyzed photosynthesis, physiology, submicroscopic structure to explore the response mechanism of A. giraldii to different light intensity. Light was the main influencing factor to photosynthetic rate.During morning and afternoon periods,the Pn of the CK group reduced by stomatal limitation and non stomatal limitation factors respectively. While during 14：30-18：30 period, the Pn of A₁ and A₂ groups reduced by non stomatal limitation factors.LSP, LCP and Rd of A₁ and A₂ groups were significantly lower than those of CK group;The content of SS and SP of A₁ and A₂ groups were lower than those of CK group. The content of Pro of CK group were significantly higher than those of group A₂.The activities of SOD and POD of them was higher than that of CK group,CAT activity of A₁ and POD activity of A₂ were relatively higher In their respective free radical scavenging system. Starch grain increased and base grana declined in the chloropalst of those group CK. The study results indicated that response mechanism of different shading conditions of A. giraldii under field cultivation conditions. Its could effectively adapt to environmental changes of the home cultivation,which provided a reference for ensuring yield and quality.

Hypoglossal nerve stimulation for treatment of obstructive sleep apnea (OSA): a primer for oral and maxillofacial surgeons / 대한악안면성형재건외과학회지

The prevalence of obstructive sleep apnea (OSA) is estimated to be 1–5% of the adult population world-wide, and in Korea, it is reported at 4.5% of men and 3.2% of women (Age 40 to 69 years old). Active treatment of OSA is associated with decrease in insulin resistance, cardiovascular disease, psychosocial problems, and mortality. Surgical treatment of OSA has evolved in the era of neuromodulation with the advent of hypoglossal nerve stimulation (HGNS). We share this review of HGNS with our maxillofacial surgical colleagues to expand the scope of surgical care for OSA.

Evaluation of the wound-healing activity and anti-inflammatory activity of aqueous extracts from Acorus calamus l.

In folklore medicine, Acorus calamus has been used as a wound-healing agent for thousands of years; however, there have been few scientific reports on this activity so far. Now, we explored deeply the wound-healing effect of aqueous extracts from the fresh roots and rhizomes of A. calamus in vivo, as well as anti-inflammatory activity in vitro, so as to provide scientific evidence for the traditional application. The wound-healing effect was determined by the image analysis techniques and the histological analysis in the excisional wounding test, and the anti-inflammatory activity was evaluated by the real-time RT-PCR techniques in the lipopolysaccharide-induced RAW 264.7 cells test. Aqueous extracts, administered topically at the dose range from twice to thrice in a day, could enhance significantly the rate of skin wound-healing. Moreover, the extracts could effectively inhibit the mRNA expressions of inflammatory mediators induced by lipopolysaccharide in RAW 264.7 cells. These results showed significantly the wound-healing activity of aqueous extracts in the animal model of excise wound healing, and anti-inflammatory activity in vitro.

Comparison of local immune microenvironment between liver-metastasis colorectal cancer and non-liver-metastasis colorectal cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the difference of local immune microenvironment in primary tumors between liver-metastasis and non-liver-metastasis cohort in stage III to IIII colorectal cancer patients.</p><p><b>METHODS</b>Tumor samples from 167 patients of colorectal cancer were harvested, who received tumor resection for the first time in The First Affiliated Hospital of Sun Yat-sen University from 2000 to 2005. Patients were divided into two groups according to liver metastasis or not. Expressions of 18 immune markers, including CD3, CD4 and CD8 were examined and quantified by immunohistochemistry staining.</p><p><b>RESULTS</b>No significant differences of gender, age, BMI, tumor differentiation, pathology type and preoperative CEA level were found between the two groups. The expressions of CD8, CD45RO, IL-17, tryptase and FAS were lower in liver-metastasis group as compared to non-liver-metastasis group (all P<0.05).</p><p><b>CONCLUSIONS</b>Decrease of the number of T lymphocyte and mast cell may play an important role in local infiltration of immune microenvironment of stage III to IIII colorectal cancer with liver metastasis.</p>

Syncope with spontaneous coronary artery dissection in an elderly woman / 中华医学杂志(英文版)

Diagnosis of spontaneous coronary artery dissection (SCAD) is challenging because of its rarity and uncertain etiology. It frequently occurs in young women during pregnancy and in the postpartum period, and rarely found in elder women with no history of cardiovascular disease or coronary risk factors. In this article we report a case of SCAD in a 75-year-old woman without traditional cardiovascular risk factors who presented with syncope and mild chest discomfort. There were no abnormal electrocardiographic changes and no elevated cardiac enzymes were detected. Computed tomography of brain revealed nothing abnormal. Coronary artery disease was suspected. Coronary angiogram revealed dissection in the middle left circumflex artery. The patient underwent percutaneous transluminal coronary angioplasty and was free of symptoms at 6-month follow-up. Our report suggests that emergency coronary angiography is indicated if syncope caused by coronary artery disease is suspected.